Composite

Part:BBa_K5519004:Design

Designed by: Latifat Abdulsalam   Group: iGEM24_KFUPM-Saudi-Arabia   (2024-09-16)


AB_pTac_BborAMP1_TEVcut_GFP_His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1093
    Illegal NotI site found at 1235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1093
    Illegal BamHI site found at 1087
    Illegal XhoI site found at 1244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1093
    Illegal NgoMIV site found at 1018
    Illegal AgeI site found at 487
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this construct is meant to be expressed in Escherichia coli, the sequence of BborAMP1 was codon optimized for expression in E.coli.


Source

BborAMP1 comes from the genome sequence of the thermophilic bacteria Brevibacillus Borstelensis AK1 APBN01000001.1

References